Reversible terminator sequencing at UCSF

Definition
is a DNA sequencing which allows sequence identification by relying on use of DNA polymerase and reversible terminator. The methods requires immobilization of genomic DNA fragment onto a surface and a specific clonal amplification step known as bridge PCR. Reliance on reversible terminator allow cycles of DNA chain extension by DNA polymerase and imaging without the need of electrophoretic separation of newly synthesized DNA fragment as with Sanger sequencing.
Synonyms
Solexa sequencing
Categories
→ Reversible terminator sequencing
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Mission Bay

Gladstone Genomics Core

Gladstone Institutes, Mission Bay
Contact: Nathasha Carli 415-734-2799 genomics-lab@gladstone.ucsf.edu

  • Illumina MiSeq sequencing
  • Illumina NextSeq500 sequencing