Library preparation at UCSF
- Definition
- is a process which results in the creation of a library from fragments of DNA using cloning vectors or oligonucleotides with the role of adaptors.
- Synonyms
- Library construction
- Categories
- Material processing → Reagent library preparation → Library preparation
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Mission Bay
Gladstone Genomics Core
Gladstone Institutes,
Mission Bay
Contact: Nathasha Carli
415-734-2799
genomics-lab@gladstone.ucsf.edu
- ChIP-seq library preparation
- Fluidigm C1 Single Cell Autoprep System
- NGS (Next-Generation Sequencing) library preparation
- RNA-sequencing library preparation
- RRBS (Reduced Representation Bisulfite Sequencing) library preparation
- Single-cell RNA-seq: 10x Genomics Single Cell 3’ v2
- Single-cell RNA-seq: 10x Genomics Single Cell V(D)J
- Small RNA-seq library preparation
Mission Bay
IGI CRISPR Screening Core
Innovative Genomics Institute,
Mission Bay
Contact: Chong Park
(415) 514-1219
Chong.Park@ucsf.edu
- Custom guide RNA library cloning
- Library distribution: CRISPRi, CRISPRa, other specialized
Mission Bay
UCSF Functional Genomics Core
Medicine,
Mission Bay
Contact: Andrea Barczak
415-514-4373
andrea.barczak@ucsf.edu
- ChIP-Seq library construction
- NGS library construction
- RNA-Seq library construction
- small RNA-Seq library construction
Parnassus
IHG Genomics Core Facility
Institute for Human Genetics,
Parnassus
Contact: Eunice Wan
(415) 514-3879
Eunice.Wan@ucsf.edu
- Library construction for exome sequencing