Total internal reflection fluorescence instrument at UCSF

Definition
An instrument used to observe single molecule fluorescence at surfaces and interfaces. The technique is commonly employed to investigate the interaction of molecules with surfaces.
Synonyms
TIRF
Categories
→ Total internal reflection fluorescence instrument
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Mission Bay

Nikon Imaging Center

Biochemistry and Biophysics, Mission Bay
Contact: Kurt Thorn 415-326-4566 kurt.thorn@ucsf.edu

  • TIRF/STORM Microscope: Fully motorized Nikon Ti-E microscope with perfect focus, TIRF Illuminator (laser lines: 405 nm, 488 nm, 561 nm, 647 nm), EMCCD cameras, Nikon N-STORM software. Capable of video rate TIRF microscopy and super-resolution imaging using PALM/STORM to generate images with resolution down to 20 nm.