Nucleotide sequencing, high-throughput at UCSF
- A DNA sequencing technique that became commercially available in 2004 and is used by specific commercial platforms that embody a complex interplay of enzymology, chemistry, high-resolution optics, hardware, and software engineering. These instruments allow highly streamlined sample preparation steps prior to DNA sequencing, which provides a significant time savings and a minimal requirement for associated equipment in comparison to the highly automated, multistep pipelines necessary for clone-based high-throughput sequencing. Each technology amplifies single strands of a fragment library and perform sequencing reactions on the amplified strands. The fragment libraries are obtained by annealing platform-specific linkers to blunt-ended fragments generated directly from a genome or DNA source of interest. Because the presence of adapter sequences means that the molecules then can be selectively amplified by PCR, and no bacterial cloning step is required to amplify the genomic fragment in a bacterial intermediate as is done in traditional sequencing approaches.
- Next generation sequencing, Genome sequencing, High throughput DNA sequencing, High throughput nucleotide sequencing, NGS, Next gen, Next gen sequencing, Next generation sequencing of target genes, Second generation sequencing, Sequencing, high-throughput nucleotide
- Molecular assay → Nucleic acid assay → DNA sequencing → Nucleotide sequencing, high-throughput
- Solexa sequencing