NGS at UCSF

Definition
A DNA sequencing technique that became commercially available in 2004 and is used by specific commercial platforms that embody a complex interplay of enzymology, chemistry, high-resolution optics, hardware, and software engineering. These instruments allow highly streamlined sample preparation steps prior to DNA sequencing, which provides a significant time savings and a minimal requirement for associated equipment in comparison to the highly automated, multistep pipelines necessary for clone-based high-throughput sequencing. Each technology amplifies single strands of a fragment library and perform sequencing reactions on the amplified strands. The fragment libraries are obtained by annealing platform-specific linkers to blunt-ended fragments generated directly from a genome or DNA source of interest. Because the presence of adapter sequences means that the molecules then can be selectively amplified by PCR, and no bacterial cloning step is required to amplify the genomic fragment in a bacterial intermediate as is done in traditional sequencing approaches.
Synonyms
Next generation sequencing, Genome sequencing, High throughput DNA sequencing, High throughput nucleotide sequencing, Next gen, Next gen sequencing, Next generation sequencing of target genes, Nucleotide sequencing, high-throughput, Second generation sequencing, Sequencing, high-throughput nucleotide
Categories
→ NGS
Subcategories
Solexa sequencing
See also: Solexa sequencing
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Mission Bay

Cancer Center Computational Biology Core

Helen Diller Family Comprehensive Cancer Center, Mission Bay
Contact: Adam Olshen 415-514-9406 olshena@biostat.ucsf.edu

  • High-throughput sequencing -- DNA-Seq, RNA-Seq, Exome-Seq, ribosome profiling
Mission Bay

Gladstone Genomics Core

Gladstone Institutes, Mission Bay
Contact: Nathasha Carli 415-734-2799 genomics-lab@gladstone.ucsf.edu

  • Consultation on Next-Generation Sequencing experiment design
Parnassus

IHG Genomics Core Facility

Institute for Human Genetics, Parnassus
Contact: Eunice Wan (415) 514-3879 Eunice.Wan@ucsf.edu

  • Illumina HiSeq 3500 and 4000
  • High-throughput sequencing