Mouse Genetics Core

This core facility is designed to facilitate the creation of new mutant mouse strains for diabetes-related research. The main emphasis of the core is to provide diabetes investigators access to services that are not readily available from commercial entities and that would require prohibitive investment of time and effort for them to establish in their own laboratories. Thus, the core provides expertise in the areas of gene-targeting in mouse embryonic stem (ES) cells and in microinjection of mouse embryos. The core is focused on the importation and use of cell lines, reagents and other resources that will facilitate investigations into the mechanisms that cause diabetes. Please see our homepage for more information including pricing.

Services Provided:

• Microinjection of DNA into fertilized mouse oocytes to create transgenic mice
• Microinjection of ES cells into mouse blastocysts to create chimeric mice
• Gene targeting in ES cell
• Culture and transfection of ES cells
• Selection, isolation and freezing of clones of ES cells
• Preparation of DNA from clones of ES cells
• Digestion of DNA and preparation of Southern blots for screening cloned ES cells
• Expansion of targeted ES cells from 96-well plates
• Rederivation of mouse strains by embryo transfer

Location
UCSF
Department of Microbiology & Immunology
513 Parnassus Avenue
Box 0414 - HSE Room 1101E
San Francisco, CA 94143-0414

Facility Contact
Nigel Killeen, PhD
Faculty Director
Phone: 415.502.5495

Facility Homepage

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