Library construction at UCSF

Definition
is a process which results in the creation of a library from fragments of DNA using cloning vectors or oligonucleotides with the role of adaptors.
Synonyms
Library preparation
Categories
→ Library construction
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Mission Bay

Gladstone Genomics Core

Gladstone Institutes, Mission Bay
Contact: Nathasha Carli 415-734-2799 genomics-lab@gladstone.ucsf.edu

  • ChIP-seq library preparation
  • Fluidigm C1 Single Cell Autoprep System
  • NGS (Next-Generation Sequencing) library preparation
  • RNA-sequencing library preparation
  • RRBS (Reduced Representation Bisulfite Sequencing) library preparation
  • Single-cell RNA-seq: 10x Genomics Single Cell 3’ v2
  • Single-cell RNA-seq: 10x Genomics Single Cell V(D)J
  • Small RNA-seq library preparation
Mission Bay

IGI CRISPR Screening Core

Innovative Genomics Institute, Mission Bay
Contact: Chong Park (415) 514-1219 Chong.Park@ucsf.edu

  • Custom guide RNA library cloning
  • Library distribution: CRISPRi, CRISPRa, other specialized
Parnassus

IHG Genomics Core Facility

Institute for Human Genetics, Parnassus
Contact: Eunice Wan (415) 514-3879 Eunice.Wan@ucsf.edu

  • Library construction for exome sequencing